Exercising with insulin action

Time
21 August 2019, 13:00

Place
Aud 1, August Krogh Building, Universitetsparken 13, 2100 Copenhagen

Opponents
Associate professor Faidon Magkos (chair), Department of Nutrition, Exercise and Sports, University of Copenhagen, Denmark

Professor Niels Ørtenblad, University of Southern Denmark, Denmark

Professor Gregory Cartee, University of Michigan, USA

Supervisor
Professor Jørgen Wojtaszewski, Department of Nutrition, Exercise and Sports, University of Copenhagen, Denmark

About the thesis
Exercise is known to be beneficial in the prevention and treatment of various diseases. For instance, exercise training improves the ability of the body to regulate blood glucose levels, in part due to enhanced insulin action on glucose uptake in skeletal muscle. This is evident in both healthy individuals as well as in individuals with a reduced ability of insulin to increase glucose uptake (e.g. Type 2 diabetics).

In addition to the effects of regular exercise training, a single bout of exercise also improves insulin action, an effect confined to the prior exercised muscle. However, the underlying molecular mechanisms responsible for this phenomenon are not fully elucidated. Furthermore, factors determining the magnitude of the response both within the muscle and at a whole-body level are not completely known. Thus, the aim of the present thesis was to investigate factors important for insulin action in muscle and the enhancement by a single bout of exercise in humans.

We demonstrated that the ability of the muscle to enhance insulin action in response to a single bout of exercise was reduced after a period of regular exercise training. We also demonstrated that exhaustive exercise of a small muscle group increased insulin action in the prior exercised muscle while at the same time impairing insulin action in non-exercised muscle. Due to the large amount of inactive muscles, this resulted in reduced whole-body insulin action after a single bout of exercise.

Thus, the magnitude of the response to a single bout of exercise in muscle and at a whole-body level seems to be dependent on training status as well as the amount of inactive vs. active muscle mass engaged during exercise. Analysis of muscle biopsies confirmed previous findings in rodents that AMPK, an energy sensor of the cell, is important for mediating the effects of a single bout of exercise on insulin action.

In addition, we also used a proteomic approach (a large scale study of proteins) to evaluate the protein signature of different types of human skeletal muscle fibers as well as the remodeling in response to exercise training. This provided a huge resource for future research and understanding of muscle metabolism.

Read more: https://nexs.ku.dk/english/calendar/2019/phd_dorte-enggaard-steenberg/